The cells were allowed to attach to the bottom of the wells overnight. In the following example, HEK 293 cells were trypsinized and plated into the wells of a 96-well polystyrene tissue culture microplate at a density of 25,000 cells per well in 50 µl of culture media. The Bridge-It ® cAMP all in one assay allows for the stimulation and measurement of cAMP in adherent monolayer cells attached to the wells of a tissue culture microplate. We are always happy to help!Įxample of Bridge-It ® cAMP all in one Assay for Cells in Suspension For any other questions or comments, please do not hesitate to contact us. For bulk pricing options, please contact us.
CYCLIC AMP ASSAY TRIAL
Pricing: The 100-well kit is a one-time trial purchase. The sensitivity and performance characteristics of the Bridge-It ®cAMP designer and the Bridge-It ® all in one assay products are presented in the following Table and Figures.
No response was detected using the Bridge-It ® cAMP assay with any of these compounds within the concentration range that would be expected to occur in “real life” samples (i.e., millimolar range for AMP and ATP, and micromolar range for cGMP). ATP, AMP, and cGMP have all been tested for selectivity using the cAMP assay.
Assay Kits for Quantitative Detection of:.
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